To uncover more CRMs, Kroemer and his team now tested 200 compounds that belong to the same class of substances as spermidine and resveratrol. They first examined which of these substances show cellular reactions characteristic of elevated autophagy in rat and human cell cultures, while not being toxic to the cells. An agent called 3,4-dimethoxy chalcone (3,4-DC) stood out as the best candidate. A closer look at the exact mode of action of 3,4-DC revealed that it induces autophagy through a different pathway than spermidine and resveratrol.

https://www.sciencedaily.com/releases/2019/10/191021111842.htm